Toleration of Lucilia cuprina (Diptera: Calliphoridae) Eggs to Agitation Within Insect Saline
Abstract
Microorganisms have an ability to attach themselves to surfaces, where they then multiply and form a protective biofilm, which protects the microorganisms from outside influences, including chemicals for sanitation. This makes the cleaning of fly eggs for maggot therapy difficult. The purpose of this study was to test the toleration of Lucilia cuprina (Wiedemann) (Diptera: Calliphoridae)eggs for agitation, which is used to degrade biofilms. Lucilia cuprina eggs were washed with insect saline and treated by undergoing five minutes of agitation. It was found that there was a significant difference between the control group, which did not undergo any agitation, and the treatment group, which did. This knowledge is useful for further testing of the extent to which agitation impacts survivability of the eggs and the debridement of the biofilm.
References
Chai, Z., Wang, J., Tao, S., & Mou, H. 2014. Application of bacteriophage-borne enzyme combined with chlorine dioxide on controlling bacterial biofilm. Food Science and Technology, 59(2), 1159-1165.
Hughes, K.A., Sutherland, I.W., Jones, M.V. 1998. Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase. Microbiology, 144, 3039—3047
Orgaz, B., Kives, J., Pedregosa, A.M., Monistrol, I.F., Laborda, F., SanJose, C. 2006. Bacterial biofilm removal using fungal enzymes. Enzyme and Microbial Technology, 40, 51—56
Parnés, A., Lagan, K.M. 2007. Larval therapy in wound management: a review. Int. J. Clin. Pract.61: 488—493.
Pringle, J. W. S. 1938. Proprioception in insects. I. A new type of mechanical receptor from the palps of the cockroach. J. Exp. Biol. 15, 101-13.
Thyssen PJ, Nassu MP, Nitsche MJT, Leite DS. 2013. Sterilization of immature blowflies (Calliphoridae) for use in larval therapy. J. Med. Med. Sci. 4(10):405-409
Whitaker, I. S., C. Twine, M. J. Whitaker, M. Welck, C. S. Brown, and A. Shandall. 2007. Larval therapy from antiquity to the present day: mechanisms of action, clinical applications and future potential.Postgrad. Med. J. 83: 409—413
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