A comparison of follicle mite (Demodex sp.) collection methods.

Authors

  • Madison Anne McClintock Texas A&M University Dr. Brundage's class
  • Keala Kuaiwa
  • Jake Kindle
  • Alex Armendarez
  • Madeline Maguire
  • Sydney Tejml
  • Brandon Mason
  • Hunter Gurley

Keywords:

Demodex, hair follicle mites, dermatitis

Abstract

Demodex is a genus of microscopic mites that live on human hosts, feeding on sebum and dead skin. They are classified as arachnids with an elongated abdomen that allows them to dwell in pores and hair follicles. The mites do not commonly cause any problems but a diseased state may arise if their population suddenly increases, leading to rosacea, blepharitis, and general dermatitis. Collecting the mites can be difficult and two collection methods were compared. The first method was spreading super glue onto a microscope slide and adhering it to the forehead for five minutes. The second was done by scraping the area on the forehead and transferring the debris onto another slide. For the eight participants, only one Demodex was found overall, using the super glue method. Our study concluded that, although mostly ineffective, the super glue method was best to collect and observe Demodex from human skin.

References

Aylesworth, R., and J. C. Vance. 1982. Demodex folliculorum and Demodex brevis in cutaneous biopsies. J. Am. Acad. Dermatol. 7(5): 583-589.

Bonnar, E., P. Eustace, and F. C. Powell. 1993. The Demodex mite population in rosacea. J. Am. Acad. Dermatol. 28(3): 443-448.

Elston, D. M. 2010. Demodex mites: Facts and controversies. Clin. Dermatol. 28(5): 502-504.

Enginyurt, O., U. Karaman, F. Cetin, and A. Ozer. 2015. The prevalence of Demodex species and its relationship with the metabolic syndrome in women of Malatya Province, Turkey. Jundishapur J Microbiol. 8(10): e24322.

Forton, F., and B. Seys. 1993. Density of Demodex folliculorum in rosacea: a case-control study using standardized skin-surface biopsy. Brit. J. Dermatol. 128(6): 650-659.

Lacey, N., K. Kavanagh, and S. C. G. Tseng. 2009. Under the Lash: Demodex mites in human diseases. Biochem. Lond. 31(4): 2-6.

Mullen, G. R., and L. A. Durden. 2019. Medical and Veterinary Entomology. London: Academic Press, an imprint of Elsevier. Cambridge, Massachusetts, United States.

Murphy O., V. O'Dwyer, and A. Lloyd-McKernan. 2018. The efficacy of tea tree face wash, 1, 2-Octanediol and microblepharoex foliation in treating Demodex folliculorum blepharitis. Contact Lens Anterio. 41(1): 77-82.

Powell, F. 2015. Demodex: Exploring its role in rosacea. https://practicaldermatology.com/articles/2015-oct/demodex-exploring-its-role-in-rosacea

Rather, P.A., and I. Hassan. 2014. Human Demodex Mite: The Versatile Mite of Dermatological Importance. Indian J. Dermatol. 59(1): 60-66.

Yun C.H., Yun J.H., Baek J.O., Roh J.Y., Lee J.R. 2017. Demodex Mite Density Determinations by Standardized Skin Surface Biopsy and Direct Microscopic Examination and Their Relations with Clinical Types and Distribution Patterns. Ann. Dermatol. 29(2):137-142.

Published

2021-01-27