Prevalence of Anaplasma (A.) Genus in Rhipicephalus sanguineus (L.) (Acari: Ixodidae) Found in Shelter


  • Frida Nohemi Cano


Animal shelters are known for being an excellent place for various diseases to be transmitted among dogs, since there is a large volume of animals enclosed in a relative small space. Rhipicephalus sanguineus (L.) (Acari: Ixodidae) commonly known as the brown dog tick is unusual among ticks, because it can complete its entire life cycle inside making it the perfect vector for diseases. In this study we looked at the prevalence of one of the main vector borne disease in the dog population of Texas, anaplasma. Three hundred and nine ticks used for the experiment were collected from six shelters around Texas. This ticks were identified to species. Three hundred and eight were identified to be Rhipicephalus sanguineus, only one Dermacentor Variabilis (S.) (Acari: Ixodidae)  was collected. DNA was extracted from 90 ticks; the DNA was analyzed using polymerase chain reactions (PCR). Positive samples were sent to a gene bank for sequencing.  Eleven samples came back as positive for Anaplasma genus. We concluded that the prevelansce of anaplasma might be much higher than previously expected and that more studies have to be conducted on the subject. 


Holt, David E., Michael R. Mover, and Dorothy Cimino Brown. "Serologic prevalence of antibodies against canine influenza virus (H3N8) in dogs in a metropolitan animal shelter." Journal of the American Veterinary Medical Association 237.1 (2010): 71-73.

Jensen, J., et al. "Anaplasma phagocytophilum in dogs in Germany." Zoonoses and public health 54.2 (2007): 94-101.

Yabsley, Michael J., et al. "Prevalence of Ehrlichia canis Anaplasma platys Babesia canis vogeli Hepatozoon canis Bartonella vinsonii berkhoffii, and Rickettsia spp. in dogs from Grenada." Veterinary parasitology 151.2 (2008): 279-285.

Ravnik, Urska, et al. "Anaplasmosis in dogs: The relation of haematological, biochemical and clinical alterations to antibody titre and PCR confirmed infection." Veterinary microbiology 149.1 (2011): 172-176.

Villeneuve, Alain, et al. "Seroprevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, Ehrlichia canis, and Dirofilaria immitis among dogs in Canada." The Canadian Veterinary Journal 52.5 (2011): 527.

L.A. Durden, J.E. Keirans Nymphs of the Genus Ixodes (Acari: Ixodidae) of the United States: Taxonomy, Identification Key, Distribution, Hosts, and Medical/Veterinary Importance Thomas Say Publications in Entomology, Entomological Society of America (1996)

J.E. Keirans, C.M. Clifford The genus Ixodes in the United States: a scanning electron microscope study and key to the adults J. Med. Entomol. Suppl., 2 (1978), pp. 1—149

D.E. Sonenshine Ticks of Virginia Virginia Polytechnic Institute and State University, College of Agriculture and Life Sciences, Blacksburg, VA (1979)

Krakowetz, Chantel N. "Two Anaplasma phagocytophilum Strains in Ixodes scapularis Ticks, Canada." Atlantic 4.71: 1-5. Krakowetz, Chantel N. "Two Anaplasma phagocytophilum Strains in Ixodes scapularis Ticks, Canada." Atlantic 4.71: 1-5.

Beall, Melissa J., et al. "Serological and molecular prevalence of Borrelia burgdorferi, Anaplasma phagocytophilum, and Ehrlichia species in dogs from Minnesota." Vector-Borne and Zoonotic Diseases 8.4 (2008): 455-464.